Thickness of cumulus cell layer is a significant factor in meiotic competence of buffalo oocytes
نویسندگان
چکیده
منابع مشابه
Effects of cysteamine during in vitro maturation on viability and meiotic competence of vitrified buffalo oocytes
The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...
متن کاملIn Vitro Maturation and Fertilization of Denuded Buffalo Oocytes with Cumulus Cell Monolayers
Buffalo ovaries were collected from a slaughter house and cumulus cells oocytes complexes (COCs) were recovered for preparation of 2-D, 4-D, 6-D and 8-D monolayer. Cumulus cells were stripped off by repeated pipetting into TCM-199 medium supplemented with 10% FCS. Approximately 2x106/ml cumulus cell were transferred in 100 μl drops of culture medium and incubated in humidifi ed atmosphere of 5%...
متن کاملInhibition of meiotic maturation in growing pig oocytes by factor(s) from cumulus cells.
Total inhibition of germinal vesicle breakdown (GVBD) was observed in growing pig oocytes (internal diameter 80, 90 and 100 microns) when they were cultured in a medium conditioned by cumulus oocyte complexes (COCs) of fully grown oocytes. In denuded growing oocytes, only partial inhibition was observed. The inhibitory effect was fully reversible. The addition of heparin (300 IU/ml) could overc...
متن کاملEffect of Recombinant Human Follicle Stimulating Hormone on Meiotic Competence of In Vitro Grown Nili Ravi Buffalo Oocytes
In the present study, the response of Nili Ravi buffalo oocytes to recombinant human follicle stimulating hormone (rhFSH) (Organon) on meiotic maturation in vitro was examined. Oocytes were matured in vitro in medium containing either 0 or 0.05 IU/ ml rhFSH and the stage of nuclear maturation recorded after 24 hours. The percentage of oocytes in the control group undergoing germinal vesicle bre...
متن کاملthe effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
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ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2004
ISSN: 1229-845X
DOI: 10.4142/jvs.2004.5.3.247